Carolina NeuLog Ammonium Ion-Selective - Manual

NEULOG AMMONIUM SENSOR GUIDE
NeuLog chloride sensor
NUL-240
Carolina # 369669
The NeuLog chloride ion sensor can be used for any science experiment or activity
which can utilize chloride concentration readings such as in the fields of
Environmental Science, Ecology, Chemistry, Biology, etc.
Just a few of the thousands of possible experimental subjects that can be done with
the NUL-240 sensors are: chemical reactions, water quality, environmental health,
small animal behavior, ecological studies, and many more
The chloride sensor uses the following units of measure:
 Milligrams per liter (mg/L): SI unit of measure for density of a solid dissolved
in a liquid.
 Parts per million (ppm): The total amount of a specific molecule (chloride for
example) per million molecules in a sample.
Chloride sensor usage:
The chloride
sensor’s electrode uses a PermaFil (non-refillable), Ion-selective
electrode. The reference chamber is gel filled and sealed and therefore no reference
fill solution is required.
Usage guide:
Concentration range
1M to 5x10 -6 M
0.01 to 18,000 ppm
pH range 4 to 10 pH
Temperature range 0 to 50°C
Minimum sample size 3mL in a 50 mL beaker
Reproducibility ±4%
Electrode resistance 1 to 4 megaohms
Required equipment:
 Wash bottle with distilled or deionized water.
 Several clean beakers.
 1 mL, 10 mL, and 100 mL pipettes.
Technical notes:
 All standard solutions should be prepared fresh.
 The standards should be at the same temperature as the sample.
 Use ISA in all solutions.
Note: ISA is added to all solutions to ensure the samples and standards
have the same ionic strength. The ratio is 1:50
Electrode preparation:
1. Remove the protective cap encasing the chloride probe’s electrode.
CAUTION: Do not touch the PVC membrane at the end of the probe
with your fingers
2. Rinse the electrode with deionized water and blot dry. Be sure not to rub as
this will harm the sensor.
3. Soak the electrode in deionized water for 10 minutes, then in a diluted
chloride standard solution (either 0.0001 M or 1 ppm) until ready for use.
Calibration:
Be sure to calibrate the chloride sensor once daily during periods of regular usage.
1. Prepare the electrode as described in the “Electrode preparation” guide
above.
2. Place 97 mL of deionized water into a 150 mL beaker.
3. Add 2 mL of Ionic Strength Adjustor (ISA) (comes with sensor).
4. Rinse the electrode with deionized water and blot dry and place in the 150
mL beaker.
5. Pipette 1 mL of 1000 ppm chloride standard solution (comes with sensor)
and stir thoroughly.
6. In the NeuLog software, once the sensor has been detected, click on the
chloride sensor icon.
7. After a stable reading has been reached, click on the calibrate button, or
hold the “Start/Stop” button located on the sensors faceplate down for 4
seconds.
8. Rinse electrode once more with deionized water and blot dry.
9. The chloride sensor is now calibrated and ready for use.