Calibration, Add new classification channel calibration lots – Luminex 100 User Manual Version 1.7 User Manual

Page 42

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Luminex 100 User Manual Version 1.7

x

MAP Technology

5 - 2

PN 89-00002-00-063 Rev. A

The Luminex Main window has six components: Title bar, Menu bar,
Tool bar, Startup display, Status bar, and System monitor.

The Title bar shows the name of the software, and changes
depending on what section of the software you are using.

The Menu bar contains commands to set up the Luminex 100
analyzer and perform data acquisition. See Chapter 6 for more detail.

The Tool bar, also discussed in greater detail in Chapter 6, contains
icons for commands that are frequently used when running the
Luminex software.

The Startup display shows icons for many frequently-used
commands. You use most of these commands when starting the
system, acquiring data, and shutting the system down.

The Status bar shows the status of current software events.

The System monitor displays Device and Calibration status.

Calibration

You should perform Luminex 100 analyzer calibration after system
installation, instrument service, whenever the system is moved, and
monthly with routine use. For optimal performance, calibrate the
instrument daily as part of your startup routine and whenever the d
Cal Temp temperature shown on the system monitor panel indicates
a change of more than 3 degrees Celsius (

°

C). The calibrators are

xMAP microspheres with known light-scattering properties and
known fluorescent intensities in the reporter (RP1), classification 1
(CL1), and classification 2 (CL2) wavelength ranges. The calibration
process uses these xMAP microspheres to adjust voltage settings for
optimal and consistent microsphere classification and reporter
readings. Current calibrated settings automatically apply to any new
session.

Add New Classification
Channel Calibration Lots

Before you perform an initial calibration for the analyzer, and
whenever you receive a new supply of xMAP calibration
microspheres, enter the product and lot numbers and the calibration
target values.

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