Luminex 100 User Manual Version 1.7 - Manual (Page 65)

x MAP Technology Data Acquisition
PN 89-00002-00-063 Rev. A 6 - 15
We recommend using at least 100 events per microsphere set. This
guarantees that each bead set selected acquires 100 events before
completing sample acquisition. If each selected bead set does not
acquire this number of events, you see a warning that not enough
bead events were acquired. You can choose to keep the data or
disregard it. Because of this option, when selecting bead sets on the
Bead Set tab, you should choose only the bead sets present in your
sample. If you select bead sets that are not present, the analyzer
continues to acquire, trying to reach the number of events per bead
for bead sets that are not in the sample.
If you choose total events instead of events per bead, the analyzer
stops acquiring data as soon as it acquires the specified number of
beads within any combination of bead sets. This means that some
bead sets may not have a minimum number of events.
Override Histogram Gates: Check this box to set known gate
values.
Min events: Set the minimum events needed for display on the
graphs and results table. When set to a number greater than 0, the
analyzer does not display data for bead sets not generating events
equal to or less than this value. Enter 0 to display all events. The
minimum events must be less than the number you entered in the
events field.
Samples: Enter the number of tubes or wells to be analyzed. The
maximum number of samples you can enter is 96 if you are using an
Luminex XYP, and 100 if you are not using an Luminex XYP.
Sample size: Set this to the amount in µL that you want the analyzer
to take up for each sample. You can enter a number between 10 µL
and 200 µL. We recommend a sample size of 25 µL or larger.
Note: To avoid air uptake, make sure that your sample well contains
at least 25 µL of sample in addition to the sample size, so that at least
25 µL of sample remains in the sample well after acquisition.
Flow rate: Set the flow rate to Slow, 30 µL/minute; Medium, 45 µL/
minute; or Fast, 60 µL/minute.