Luminex 100 User Manual Version 1.7 - Manual (Page 68)

Luminex 100 User Manual Version 1.7 x MAP Technology
6 - 18 PN 89-00002-00-063 Rev. A
2. Click X axis, then click on the desired parameter name.
If you select reporter 1 for the X axis parameter, the histogram
displays reporter-generated intensity data. The central collected
wavelength is 575 nm. With classification 1 or classification 2, peaks
are based on the emission wavelengths of the classification dyes
inside the microspheres; the central collected wavelengths are 658
nm and 712 nm, respectively. Select Doublet Discriminator to
display data relating to the light-scattering properties of the
microspheres.
Gates
An additional histogram feature is the ability to gate a specific
parameter before data collection. The gate filters graphical displays
and generated statistical data by eliminating particles smaller or
larger than a microsphere. Always set the X axis parameter to
doublet discriminator before setting a gate. Setting a gate on any
other parameter gives inaccurate results. We recommend setting the
gate on the primary peak of the doublet discriminator display,
thereby removing data collected from doublets or contaminants.
Figure 6-22. Gate Histogram
‹ To set a gate:
1. Set the X axis to Doublet Discriminator.
2. Right-click in the histogram; a pull-down menu appears.
3. Scroll to Gate and follow the arrow to the right.
4. Click Create. Dotted vertical lines appear in the histogram.
5. Use your mouse to drag each line where you want it.
Note: The gate that is in effect
when data is collected determines
which values are used in the result.
Applying a gate or changing a gate
for existing data does not change
your calculated values.
Gate
Boundaries
Gate
Numerical
Position