Luminex 100 User Manual Version 1.7 - Manual (Page 90)

Luminex 100 User Manual Version 1.7 x MAP Technology
7 - 10 PN 89-00002-00-063 Rev. A
3. In a 96-well microtiter plate where overall height is no more than
0.75 inches, place the appropriate alignment tool in the plate.
•
For a standard plate with flat-bottomed wells, stack two of
the larger (5.08 mm diameter) alignment disks in well A1.
• For filter-bottom plates, stack three of the larger alignment
disks into well A1.
• For a half-volume plate with flat-bottomed wells, stack two
of the smaller (3.35 mm diameter) alignment disks in well
A1.
• For a plate with conical wells, place one alignment sphere in
well A1.
• For round-bottom wells, stack 2 small disks in well A1.
4. Click Eject.
5. Place the 96-well microtiter plate on the Luminex XYP with
position A1 in the top left corner and click Retract to retract the
plate.
6. Use the 3/32 inch hexagonal allen wrench to loosen the height
adjustment locking screw. See the photo on page 7-9.
7. Click Options on the toolbar.
8. Click Setup XY, then click Setup. This dialog box appears:
Figure 7-8. XY Setup Dialog Box
9. Click Test to lower the sample probe into well A1.
10. Using the thumb wheel, raise and lower the sample probe until it
rests on, but does not apply pressure to, the top of the alignment
disk or sphere.
Note: To avoid adjusting the sample probe too low, open the
Luminex XYP door as you begin adjusting the probe height. The
plate should not bounce up or plunge down as you lower or raise
the sample arm.