Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 110)

Luminex 200 User Manual for LDS Version 1.7
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MAP Technology
8 - 8 PN 89-00002-00-150 Rev. A
The calibration microspheres
are not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
The wrong calibration lot was
chosen in the Calibration Setup
window. In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in both the
Classification Channel and
Reporter Channel menus.
The wrong wells are selected in
the XY tab in the Data
Collector software. On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.
Classification channel
calibrates, but Reporter
channel fails. Adjustments were made to
plates or sample probe height
after Classification calibration,
but before Reporter calibration. Readjust plates or sample probe
to the original type or position.
The calibration microspheres
are not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
Wrong calibration lot chosen in
the Calibration Setup window. In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in the
Reporter Channel menu.
Incorrect wells chosen in the
XY tab in the software. On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.