Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 112)

Luminex 200 User Manual for LDS Version 1.7
x
MAP Technology
8 - 10 PN 89-00002-00-150 Rev. A
The wrong wells are selected in
the Setup XY tab. On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.
Slow or Unsuccessful Sample
Acquisition The air pressure is out of range. See Pressurization on page 8-3.
The sample probe is clogged. Clean the sample probe. See
page 7-4.
The sample probe is not
vertically aligned. See Adjusting the Sample Probe
Height on page 4-4.
Air is present in the system. Run three Prime commands,
then resume sample acquisition.
You are using an incorrect bead
set. In Settings window, make sure
that the correct bead set is
selected. If the selected bead set
is not present in every well,
select “Total” events instead of
“Per Bead” events.
The Acquisition volume is set
too high. Set the acquisition volume to at
least 25 µL less than the actual
volume in your wells. This
setting lets the analyzer acquire
sample more efficiently with
less chance of acquiring air.
The xMAP microspheres are not
fully suspended. Vortex or pipette your samples
up and down to ensure that the
beads are present in the
solution.
You are using photobleached
microspheres. Replace photobleached
microspheres with a fresh lot of
microspheres