Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 114)

Luminex 200 User Manual for LDS Version 1.7
x
MAP Technology
8 - 12 PN 89-00002-00-150 Rev. A
Calibration microspheres
classify too high You may be using
photobleached calibration
microspheres. Replace the microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
xMAP microspheres hit
toward the lower right of the
region You may be using
photobleached xMAP
microspheres. Replace microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
Scattered beads There is air in the system. Verify sample probe height, run
2-3 Alcohol Flush commands.
There is no sheath fluid in the
sheath bottle. Make sure there is sheath fluid
in the sheath bottle. Prime the
system until all air is out of the
system.
Microspheres appear as a long
diagonal line The xMAP microspheres have
agglutinated Add additional detergent to the
assay buffer. For example,
add.02% to 0.1% of Tween-20,
Titron
®
X 100, or SDS.
The solvent is incompatible. See the Technical Support
website - http://
www.luminexcorp.com - for a
list of incompatible solvents. If
the solvent you are using is
listed on the website, switch
solvents.
You are using incompatible
sheath fluid. Use only Luminex sheath fluid
in the Luminex analyzer. Other
fluids can damage your system.