Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 117)

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MAP Technology Troubleshooting
PN 89-00002-00-150 Rev. A 8 - 15
1. Click Warmup on the toolbar. Wait 30 minutes for the warmup
to complete.
2. On the Analyzer menu, click Prime. Prime the system twice.
3. On the Analyzer menu, click Backflush. Perform this command
twice.
4. From the Analyzer menu, click Alcohol flush. Use 70%
isopropanol or 70% ethanol to flush the system.
5. Calibrate the system.
6. Vortex the xMAP calibration (CAL1) and xMAP reporter
(CAL2) calibration bead vials. Put at least five drops of the
CAL1 in the new 96-well microtiter plate using wells A1, B1,
and C1. Put at least five drops of CAL2 beads in wells D1, E1,
and F1. Ensure that the sample probe is properly aligned for use
with the plate chosen for use.
7. Click Options on the toolbar. The options dialog box opens.
8. On the User tab, click Browse. Select the Calibrator beadmap
then click OK. Click OK again to close the Settings dialog box.
9. Click New Session on the opening screen to open a new session.
A Settings dialog box opens.
10. On the General tab of the Settings dialog box, set the number of
events to 2000 and change the per bead to total using the drop-
down list. Set samples to 6 and sample size to 50 µL.
11. On the Bead Set tab of the Settings dialog box, click >> to select
both, then click OK to close the Settings dialog box.
12. Click the C button, located above the bar graph, so that the
acquisition of the sample displays while data is collected.
13. Click Start to run the beads on the Luminex analyzer.
14. Save the session and close the Luminex Data Collector software.
The folder contains these files: <output.csv>, <results.mdb>,
<session.dpg>, <project.lpx>, and the RUN files.
15. Send the session via email for a Technical Support representative
to analyze the verification. Be sure that you include all of the
files listed in step 14 with your email. The email address is
[email protected]. If you do not have access to email