Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 14)

Luminex 200 User Manual for LDS Version 1.7
x
MAP Technology
1 - 2 PN 89-00002-00-150 Rev. A
its own subset, based on its fluorescent signature. In addition, the
Luminex analyzer scans each microsphere for the presence of a
reporter fluorescence that quantifies the assay at the microsphere’s
surface.
Microtiter plates with 96 wells must be compatible with the Luminex
XYP plate holder. The following microtiter plate types are
compatible with the Luminex XYP instrument plate holder:
flatbottom, conical, round, filter bottom, or half-well plates of any
color. The overall plate height must be no more than 0.75” (19 mm).
Microtiter plates with 96 wells must be compatible with the Luminex
XYP instrument heater block temperature when performing heated
assays. The heater block temperature ranges from 35°C to 55°C
(95°F to 131°F) .
The system reads the wells in column-first order. An entire column
of wells is read; that is, well A1, B1, C1, D1, and so on to the end of
the column. Then the XY Platform moves over to the next column
and reads well A2, B2, C2, D2, and so on. When placing a plate on
the plate holder, place it so well A1 is in the upper-left corner of the
holder. You can run an entire plate, a consecutive range of samples
from the plate, or a single sample from the plate.
Basic Concepts
Luminex has pioneered a versatile and robust technology for the
measurement of soluble analytes. The Luminex 200 system performs
simultaneous, discrete measurements of multiple microsphere-based
reactions from a single specimen aliquot. A clear understanding of
the concepts and functionality associated with the instrument and
xMAP microspheres contributes to greater success with this
technology. We present a brief overview of some of the basic
concepts. For more information, refer to Practical Flow Cytometry,
4th edition, by Howard M. Shapiro, M.D. (New York: Wiley-Liss
Inc., 2003).
Fluidics
There are two fluidic paths in the Luminex analyzer. The first path is
a syringe-driven mechanism that controls the sample uptake. This
mechanism permits small sample uptake volumes from small
reaction volumes. The syringe-driven system transports a user-
specified volume of sample from a microtiter plate to the cuvette.
The sample is injected into the cuvette at a steady rate for analysis.
After analysis, the sample path is purged with sheath fluid by the
second fluidics path. This process expels sheath fluid into the sample