Calibration, Add new classification channel calibration lots – Luminex 200 User Manual with LDS 1.7 Software User Manual

Page 48

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Luminex 200 User Manual for LDS Version 1.7

x

MAP Technology

5 - 2

PN 89-00002-00-150 Rev. A

The Luminex Main window has six components: Title bar, Menu bar,
Tool bar, Startup display, Status bar, and System monitor.

The Title bar shows the name of the software, and changes
depending on what section of the software you are using.

The Menu bar contains commands to set up the Luminex analyzer
and perform data acquisition. See Chapter 6 for more detail.

The Tool bar, also discussed in greater detail in Chapter 6, contains
icons for commands that are frequently used when running the
Luminex software.

The Startup display shows icons for many frequently-used
commands. You use most of these commands when starting the
system, acquiring data, and shutting the system down.

The Status bar shows the status of current software events.

The System monitor displays Device and Calibration status.

Calibration

You must perform Luminex analyzer calibration after system
installation, instrument service, whenever the system is moved,
monthly with routine use, and whenever the d Cal Temp temperature
shown on the system monitor panel indicates a change of more than
3 degrees Celsius (

°

C). For optimal performance, calibrate the

instrument daily as part of your startup routine. The calibrators are
xMAP microspheres with known light-scattering properties and
known fluorescent intensities in the reporter (RP1), classification 1
(CL1), and classification 2 (CL2) wavelength ranges. The calibration
process uses these xMAP microspheres to adjust voltage settings for
optimal and consistent microsphere classification and reporter
readings. Current calibrated settings automatically apply to any new
session.

Add New Classification
Channel Calibration Lots

Before you perform an initial calibration for the analyzer, and
whenever you receive a new supply of xMAP calibration
microspheres, enter the product and lot numbers and the calibration
target values.

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