Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 51)

x
MAP Technology System Startup
PN 89-00002-00-150 Rev. A 5 - 5
Calibrate the Luminex
Analyzer
In addition to calibrating the analyzer after installation, you can
enhance optimal performance by calibrating the instrument daily as
part of your startup routine and whenever the d Cal Temp
temperature shown on the system monitor panel indicates a change
of more than 3 degrees Celsius (
° C).
‹
To calibrate the Luminex analyzer:
1. Turn on the Luminex analyzer. Click Warmup on the main
screen, then click OK on the dialog box that appears. After the
30-minute warmup, go on to the next step.
2. Click Prime on the main screen. Click OK when you want to
begin the prime cycle.
3. Click Options on the toolbar and ensure that the setting for
alcohol flush and wash is set to Reservoir, and that the wells
selected for CAL1 and CAL2 correspond to the wells in the plate
that you want to use for calibration.
4. Vortex the xMAP calibrator containers to ensure homogeneity
(CAL1 and CAL2).
5. Load a microtiter plate with at least five drops of xMAP
classification microspheres (CAL1) and at least five drops of
xMAP reporter calibration microspheres (CAL2) into the wells
you designated.
Figure 5-6. 96-well Plate. Well A1 and B1 Filled
6. Click Eject on the toolbar and place the plate on the plate holder
with well A1 in the top, left-hand corner.
Note: To select a different well
location, click Options on the
toolbar. Select the Setup XY tab,
then click on the drop-down arrow
next to the entry cell for the
calibrator, then click on the well
location.
Note: Do not dilute the xMAP
calibrator reagents. Limit exposure
to light!
Note: When dispensing calibration
and control microspheres, hold the
bottle upside down at a 90-degree
angle to the microtiter plate to
ensure that you are getting
accurate drop volume.
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
Place reporter
calibrator in a
well (i.e., B1).
Place classification
calibrator in a well
(i.e., A1). You may
assign different
well locations, if
desired.