Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 27)

NOTE: See the Defining the System Initialization section for more
information.
Calibrator xMAP beads are used to normalize the settings for the reporter channel, all
classification channels, and the doublet discriminator channel. Verification xMAP beads are
used to verify calibration and optical integrity of the system.
If the system is not fully calibrated, a warning message opens.
Once calibrated, the values remain until you recalibrate. You can track system calibration and
verification results through the Calibration and Verification report.
Luminex ® recommends that you calibrate your system once a week. In addition, recalibrate
the system if any of the following things occur:
• The delta calibration temperature exceeds ± 5°C.
• You move the instrument.
• You experience sample acquisition problems.
• The instrument undergoes hardware maintenance, such as replacement of a part.
Luminex ® recommends that you verify system performance on a daily basis.
The daily system initialization routine can be set to include calibration and or verification
under the Admin page, System Setup tab, Maintenance Options section.
Before you can calibrate or verify the system, you must import FLEXMAP 3D ® calibrator and
verification bead lot information. Do this using the Lot Management tab of the Maintenance
page. This information is available on the CD that accompanies the Performance Verification
Kit and the Calibration Kit, and also available on the Luminex website at http://
www.luminexcorp.com/Support/index.htm.
NOTE: Ensure that the Luminex analyzer lasers are warmed up and the
probe height is set correctly before calibrating the system. Do not
move the system waste line while calibrating.
Shutting Down the Analyzer
Run the daily shutdown routine to prevent clogs and crystallization of salt in the sample
probe. Clogs and crystallization of salt in the sample probe can cause problems with
calibration, verification, and data acquisition; they can also cause sample splashing. Shut
down the system properly to ensure system integrity.
Logging Off and Exiting
To log off and exit xPONENT ® :
1. Click Logoff at the top of the page.
2. When the Confirm dialog box opens, click OK. This opens the Log In page, with Exit on
the left tab.
Introduction
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