Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 77)

• Recalculate data - Reanalyze the batch results using only the batch MFI values. The
batch MFI values are recalculated using the analysis settings and plate layout selected
in the new recalculated batch or protocol. Settings such as bead type, volume, timeout,
XY heater, and report gain have no effect on the replayed results. Because only the MFI
values are reanalyzed, no data is displayed in the dot plot.
• OK - Saves your changes.
• Cancel - Cancels your changes and closes the box.
• - Opens the Results tab. Use this tab to view the saved batch results for the selected
batch. When you click Open, the Results subtab opens.
Replaying a Batch
Replay batch uses the raw bead data files from the initial acquisition to reprocess the batch,
and creates a new batch output file. The bead data files are replayed using the analyte,
analysis settings, and plate layout selected in the new batch or protocol. Settings such as
bead type, Volume, and XY Heater have no effect.
Results from replaying a batch are generated in the usual manner, with new .lxb and .csv
files.
Replaying or recalculating a large batch can take 1 hour or more to complete. Batch replay
cannot be stopped while in progress. Allow adequate time for the operation to complete. The
operation is complete when all progress bars have disappeared.
A batch can be reprocessed multiple times. If the system crashes but the plate finished, the
data can be recovered by replaying the batch.
The initial batch data and output file always remain intact and unchanged. Each time you
replay or recalculate a batch, the system handles it as if it is a new batch, creating a separate
batch entry and output file.
If you select to replay or recalculate a batch that was originally run without a saved protocol,
you have to modify the settings on the following subtabs:
• Settings
• Analytes
• Stds & Ctrls
• Plate Layout
These subtabs appear under the Saved Batches tab. After you have completed them in
order, click Replay Batch on the Plate Layout subtab to perform the replay or recalculate
procedure.
Results Subtab
Results > Saved Batches > Results
Results Page
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