Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 91)

• Check Mark as Intra-Well Normalization Bead if you want to use the analyte as a
normalization bead.
NOTE: The normalization bead is a microsphere set that is included in the
assay as an internal control. It controls for sample variation and can
be used to normalize data between samples in a run.
• Check Use Threshold Ranges if you want to use a range for the analysis.
• Click Add Range to setup the threshold range.
• Type a name for the range in the Range Name field.
• Type low and high range values in the Low Value and High Value fields.
• Select the check box in the Inclusive column to include the value in the range, or
leave it clear to make the range value one unit higher than the low value, and one unit
lower than the high value.
• Highlight a range and click Delete Range to delete the range.
• Click OK to apply the new settings to the analyte first clicked, or Apply to All
Analytes to apply them to all the analytes in the protocol.
17. Click Next. The Plate Layout tab opens.
• To add maintenance commands, choose a command from the list. Highlight the well
that you want to apply it to, and then choose Pre Batch Routine or Post Batch
Routine. The Commands and Routines dialog box opens. If you are working with
more than one plate, click Add Plate to select plates to display in the Plate list.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
• As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can
also import a patient file by clicking Import List.
• Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.
18. Click Save.
Creating a Qualitative Assay Protocol
You define each Qualitative assay protocol using a single kit. The kit must contain one
standard. The standard is assigned a “Quali” value for each test. Controls are optional, but
are recommended for judging the acceptability of batch results.
To create a Qualitative Assay Protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Settings tab opens.
2. In the Name box, type the name of the protocol.
3. In the Version box, type the version of the protocol.
4. In the Manufacturer box, type the manufacturer information for the protocol.
5. Define settings in the Acquisition Settings section.
Protocols Page
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