Luminex xPONENT 4.2 for FLEXMAP - Manual (Page 92)

6. Define settings in the Analysis Settings section, selecting Qualitative as the analysis
type.
NOTE: The protocol for a qualitative assay contains a single standard. The
number of controls are optional.
7. Click Next. The Analytes tab opens.
8. Click the desired analytes (bead IDs) in the numbered grid. Information about the analyte
displays in a list on the right side of the grid.
9. To change the Default Analysis, click Change. The Analysis Settings dialog box
opens.
10. In the Analysis Settings dialog box, click the analysis method from the Analysis
Method list. Click OK to change the default analysis to a single analyte. Click Apply to
All Analytes to apply the selection to all analytes. Skip this step if you do not want to
change the default analysis or if you chose No Analysis on the Settings tab.
11. In the Units box, type the desired unit of measurement.
12. In the Count box, type the desired bead count for each analyte. Click Apply All.
13. Click Next. The Plate Layout tab opens.
• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the grouping to your
preferred grouping method.
• To add maintenance commands, choose a command from the list. Highlight the well
that you want to apply it to, and then choose Pre Batch Routine or Post Batch
Routine. The Commands and Routines dialog box opens.
If you are working with more than one plate, choose Add Plate. Here, you can add a
plate, change the order of the plates, and scroll through all plates.
14. Click Save.
Deleting a Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Delete. The Delete Protocol dialog box opens.
4. Click Yes.
Editing a Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Edit. The Settings tab opens.
4. Define settings and click Next. The Analytes tab opens.
5. Define analytes and click Next. The Plate Layout tab opens.
6. Define the plate layout.
7. Click Save.
xPONENT ® for FLEXMAP 3D ®
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