Luminex IS Version 2.3 - Manual (Page 104)

94 PN 89-00002-00-110 Rev. B
Luminex IS Software Manual For Version 2.3
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MAP Technology
CL1
Refers to dyes embedded in the microsphere. Also see classification
channel.
CL2
Refers to dyes embedded in the microsphere. Also see classification
channel.
classification channel
A specific range of wavelengths in which light intensity is measured.
Includes the emission of a given classification dye. Classification
channels are abbreviated as CL1 and CL2.
control microspheres, assay
Used to verify standards within the kit. Tells you that the curve or
thresholds are correct.
control microspheres, system
xMAP microspheres used to verify the calibration and optical
integrity for the Luminex 200 analyzer.
cuvette
Principal fluid pathway within the optics component of the system
through which the sample is read.
data reduction
The analysis of acquired batch data.
delta cal temperature
The difference between the current temperature of the Doublet
Discriminator APD and its temperature at your last calibration. The
system displays this value on the Diagnostics tab within the software.
DD temperature
The current temperature of the doublet discriminator avalanche photo
diode.
emission spectrum
Wavelength range that an excited fluorochrome emits when its
electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
event
Occurs when the signal processor determines that a particle is being
observed. Referred to as one bead as it passes through the laser.
excitation spectrum
Wavelength range that excites a molecule’s electrons to a higher
energy state. Expressed in nanometers (nm).
fluorescence
Light emission that occurs when the electrons of a fluorochrome
drop to a lower energy state.
fluorochrome
A fluorescent molecule.
fluorophore
See fluorochrome.
immunofluorescence
A technique which uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.