Luminex IS Version 2.3 - Manual (Page 37)

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MAP Technology Luminex 2.3 Software
PN 89-00002-00-110 Rev. B 27
Replicate
Averaging
Standard and control replicates are predefined in the template. You
define unknown sample replicates in batch setup indicated by
replicate Sample ID.
The data analysis function supports replicate sampling. It calculates
each sample as an individual sample, which is then averaged to
obtain a replicate average.
• Standards tab—displays standard and control average values.
• Samples tab—displays sample average values.
Replicate averages are displayed with AVG in the Loc (location)
column.
The AVG entry appears immediately after the wells being averaged
or at the end of the list depending on what you select under Sort
(Sequentially or Alphabetically).
If the system fails to determine the results for a replicate due to an
excessive skew of one of the samples, you can invalidate the out of
tolerance value. Use the Invalidate Standard (F4) or Invalidate
Control (F6) buttons at the bottom of the Analysis window. Be
aware that this fixes standards and controls, not patient sample. If
any of the replicate standards and/or controls are invalidated, the
“Avg” results reflects the average of the remaining standard and/or
control replicates.
The system flags the failed sample so you may calculate the replicate
set’s average without including the failed result in the equation.
You can sort the batch samples Sequentially (by the order in which
they are acquired), or Alphabetically (by sample ID). Select the
sorting method in the Sort group box.