Luminex IS Version 2.3 - Manual (Page 53)

x
MAP Technology Luminex 2.3 Software
PN 89-00002-00-110 Rev. B 43
discriminator channel. Control xMAP microspheres are used to
verify calibration and optical integrity for the system.
Calibrate the system at least once a month and:
• Following installation
• if the system is moved.
• if a part is replaced.
• if the delta calibration temperature shown on the system
monitor (on the Diagnostics tab) is more than ±3 degrees.
• if sample acquisition is problematic.
Each step in the calibration procedure usually takes less than one
minute. You must run xMAP controls after each calibration. Once
calibrated, the calibration values remain until you calibrate again.
You can track system calibration and verification results through the
Calibration Trend Report and the System Control Trend report. If
you need target value information for Calibration or Control
microspheres, you can find the information on the Luminex website
at
http://www.luminexcorp.com . Click on the Support link then
navigate to the FAQ page on the Support page.
Ensure that the Luminex analyzer lasers are warmed up and the
probe height is set correctly before calibrating the system. Do not
move the system waste line while calibrating.
You can run calibration and verification commands from the
Maintenance tab. You can import and export calibration and control
lots and reuse existing lot information for calibration and controls.
Run System xMAP
Calibrators
‹
To calibrate your system with xMAP calibrators:
1. Vortex the xMAP calibrator and control containers to ensure
homogeneity. Do not dilute xMAP calibrator or control reagents.
2. Load a microtiter plate with at least five drops of each: CAL1 in
well A1, CAL2 in well B1, CON1 in well C1, CON2 in well D1
and distilled water in well E1 through H1 to wash a total of four
times. Use different wells as necessary. To select different well
locations in the software, click on the drop-down arrow next to
the entry cell for the calibrator or control, then click in the well
location on the microtiter plate image.
3. Click Eject/Retract, then place the plate on the plate holder.
4. Fill the Luminex XYP reservoir with a solution of 70%
isopropanol or 70% ethanol.
5. Click Eject/Retract.
Note: When dispensing
calibration and control
microspheres, hold the bottle
upside down at a 90-degree angle
to the microtiter plate to ensure
that you are getting accurate drop
volume.