Luminex IS Version 2.3 - Manual (Page 72)

Luminex IS Software Manual for Version 2.3
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MAP Technology
62 PN 89-00002-00-110 Rev. B
Use plates with wells that will hold at least 185 µL (the extra 25 µL
from the sample, plus an extra 160 µL that is dispensed back into the
well following acquisition).
‹
To create a New Advanced Batch:
1. Open the Acq. Detail tab.
2. On the Acquisition Detail toolbar, click New Advanced Batch.
The Options Dialog Box opens with the General tab displayed.
See Figure 35.
.
Figure 35 Options Dialog Box—General Tab
3. Enter the Name, Description, and Operator information.
4. Edit the following information as desired:
• Sample Size - use values from 20 to 200 µL. To avoid air
uptake, we recommend that your sample well contains at least
25 µL in addition to the sample size.
• DD Gate - use values within the range of 0 to 32767.
• Timeout - use values of 0 to 400, where 0 = no timeout.
5. Click the arrow down next to 100 regions to select the desired
bead map you want to view. The available bead maps are 25, 50,
64, and 100 (default) regions. Select whether you want the Bead
Events results to be displayed as Per Bead or Total Beads. If
you select Total Beads, enter the number of total beads in the
text box.
• Select Per Bead to continue analyzing until each bead set has
met at least the minimum events as determined on the Bead
Set tab.