Luminex xPONENT 4.0 SP1 - Manual (Page 120)

Luminex xPONENT 4.O Software Manual
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1. Type a company name in the Company box, and any additional information in the Info:
box.
2. Click Import Logo and then Open after navigating to the desired file to import a
graphic for the report header.
3. Click Clear Logo to remove the logo.
4. Click Save.
Adjusting the Sample Probe
Adjust the sample probe to ensure that it drops far enough into the well to acquire sample.
NOTE: Ensure that there is no liquid in the wells or reservoirs.
1. You should adjust the probe height using row .On the Home page, click Probe and
Heater. The Probe & Heater tab opens.
2. Select a plate in the Plate Type list.
3. If you are using a filter bottom plate, place a large (5.08mm) alignment disk into a well.
For other plates, no disk is required. Ensure that the well location is selected on the
plate image, preferably A1 (A blue pin marks the location. To change the well location,
click on the desired well in the plate image).
4. Verify that the microtiter plate is not warped. Warped plates can lead to incorrect probe
height adjustment.
5. Click Eject to eject the plate holder.
6. Place the plate on the plate holder with A1 in the top left corner. Insert a plastic strip
well into the strip well holder.
7. Click Retract to retract the plate holder.
8. Type a name for the plate in the Plate Name box, or select a saved plate from the
Plate Name list.
9. In the Reservoir section, click in an off-plate location.
10.In the Strip Wells section, click on a well location.
11.Click Auto Adjust Height. The probe automatically adjusts itself to the plate you
selected.
NOTE: Click Save Plate.
12.
System Initialization
xPONENT
®
Software contains a pre-defined startup routine to prepare the analyzer for
data acquisition.This section describes warmup, calibration, and performance verification
of the system.
Warm up the lasers to prepare the optics prior to sample acquisition. The system
automatically begins warming up when you turn power on; however, you will need to use
the Warmup command if the system is idle for four hours or longer. Failure to properly
warm up the lasers will affect assay results and system performance.
• On the System Status bar, click the Laser: button if it is yellow.
or