Create new sample list, Edit sample lists, Setting up batches – Luminex xPONENT 4.0 SP1 User Manual

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Using the Software

Create New Sample List

1. Open the Samples page.
2. In the Sample Lists section, select the protocol you are using for the sample list, then

click Create New Samples. The Create Sample tab opens.

3. In the ID box, type the sample ID.
4. Type a patient first name in the First name box if desired.
5. Type a patient last name in the Last name box if desired.
6. To add a comment about the sample, type it in the Comment box; this is optional.
7. Click Save to add the sample to the Sample list.
8. To add more samples, click New. Repeat Steps 3-7 until you have added all of the

samples you want in your samples list.

9. Once you have added the all desired samples, click Close.

NOTE:

Samples can also be added via a LIS.

Edit Sample Lists

1. Open the Samples page.
2. In the Samples Lists section, choose the protocol you want to edit, then click Details.

The Edit Samples tab opens.

NOTE:

If the number in the Number of Samples column is “0”, there are
no samples attached to the protocol.

3. Click on a sample, then use the Move arrows to move it up or down in the sample list,

changing the order in which they will be acquired.

4. To add a new sample to the list, click New, then follow steps 3-7 in “Create New

Sample List” above.

5. To edit an existing sample, click the sample, then click Edit.
6. Once you have completed editing the sample list, click Close.

Setting Up Batches

A batch consists of a group of samples processed under control of a protocol. Batches
consist of protocols and samples for acquisition and can span more than one plate.
Protocols contain predefined commands that must be included in every batch acquisition.
You can also set up a custom batch that does not use a protocol.

You can group batches together as a multi-batch. Multi-batches can consist of any number
of batches that have been set up from different protocols and are processed
consecutively. Multi-batches can only be run on one plate.

Assay kit manufacturers may provide protocols in their kits, which are distributed on a CD.
Protocols typically include assay standards, controls, and maintenance commands (such
as washes or primes to acquire along with samples). Assay reagents are included in
assay kits. You must provide information about these reagents, such as lot numbers and
concentration values for the standards and assay controls.

NOTE:

It is recommended that the manufacturer assay kit controls are
analyzed with each batch.

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