Luminex xPONENT 4.0 SP1 - Manual (Page 128)

Luminex xPONENT 4.O Software Manual
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There are two main assay types: non-competitive and competitive. In a non-competitive
assay, the slope of a concentration versus Median Fluorescent Intensity (MFI) standard
curve is a positive number. That is, low concentrations result in low MFIs and high
concentrations result in high MFIs. Conversely, competitive assays generate a standard
curve with a negative slope, the endpoints of which are high MFI/low concentration on the
left, and low MFI/high concentrations on the right.
Allele Call. Compares groups of analytes in order to determine genotype. Groups can
include two, three, or four analytes for comparison. Set the call rate to determine what
ratio you want to set as the limit for the analyte comparison.
Analyze Current Batch
When you run a batch, the Current Batch tab opens. Here, you can view real-time
analysis of acquired analytes.
To view a single statistic type for all analytes click the statistic you want to view from the
Statistic list. To view all statistic types for a single analyte, click the analyte from the
Analyte list.
Click Single Step if you want to set the DD Gate during sample acquisition. Click Resume
to acquire the next sample. Clear Single Step if you want to run all subsequent samples
without pausing.
Manage Saved Batches
Replay Batch
You can replay a batch to reprocess batches multiple times. This function uses the data
stored in the run files from the initial acquisition to reprocess a batch and create a new
batch output file.
1. Open the Results page, then open the Saved Batches tab.
2. Click the batch you want to replay.
3. Click Replay. The Select Replay Mode dialog box opens. Click Replay batch.
4. In the Settings tab, type a batch name in the Batch Name box.
5. Type a description in the Description box
6. Change other parameters as needed.
7. Click Next.
8. In the Analytes tab, change the Analyte, Default Analysis, Units and Count as
needed. Click Next.
9. On the Stds & Ctrls tab, type the appropriate information in the Standard Information
and Control Information sections. The number of Standards and/or Controls in these
sections is defined on the Settings tab in the Analysis Settings section. If your batch
uses controls, enter the appropriate values for Expected Values. Click Low Value
from the Show list, and enter the low value for each analyte. Click High Value from the
Show list, and enter the high value for each analyte. Reagent information is not
required to replay a custom batch, unless you want to use the analysis feature.
10.Click Next.
11.In the Plate Layout tab, you can do the following: