Analyze saved batch – Luminex xPONENT 4.0 SP1 User Manual

Page 129

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117

Using the Software

To add well commands, choose the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve

added, and change the starting location on the plate. If you wish to run in replicate

mode, change the Replicate Count to the appropriate number and the Grouping to

your preferred grouping method.

To add maintenance commands, choose the desired command from the list. Choose
the well that you want to apply it to, and then choose Before Well or After Well. If you

are working with more than one plate, choose the Add & Change Plate drop down list.

Here, you can add a plate, change the order of the plates, and scroll through all plates.

As you add commands to your plate, they appear in the command sequence list. Here,
you can give each of your wells an ID. You can also import an ID list and move your

commands up and down in the command sequence chart.

NOTE:

Only wells that were acquired in the original batch can be
replayed.

12.Click Replay Batch. The Current Batch tab opens, where you can view the data as it

is replayed.

Analyze Saved Batch

Click Results on the xPONENT

®

toolbar, then open the Saved Batches tab. Click the

batch name, then click Open. The Results, Settings, Log, and Sample Details tabs
appear.

Click the Results tab to view statistical information about the batch. See “Sample
Details Tab” on page 51 for
information about this tab.

Click the Settings tab to view the batch settings report. See “Settings Tab” on page 49
for information about this tab.

Click the Log tab to view a log for the activity that occurred during acquisition of the
selected batch. See “Log Tab” on page 50 for information about this tab.

Click the Sample Details tab to view sample details for each sample in the batch. If
you are using the LIS package, click Transmit to transmit the data to the LIS.

Validate Standards

Your xPONENT

®

system administrator must give you privileges to validate standards if

you are using the Secure xPONENT package. All standards are assumed to be valid
unless explicitly invalidated.

1. Open the Results page.
2. Open the Saved Batches tab.
3. Click the batch name, then click Open. The Results tab opens.
4. Click the square area next to left of the standard you wish to validate, then click

Validate.

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