Luminex xPONENT 4.0 SP1 - Manual (Page 133)

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Using the Software
5. Type a new name for the edited formula in the Formula Name box.
6. Edit the values for each range in the Negative, Low Positive, or High Positive if you
selected Lum Qual in the Formulas list, or Negative, Low Positive, Moderate
Positive, or Strong Positive if you selected Adv Qual in the Formulas list. Select the
check box in the Inclusive column to include the value in the range. If you clear the
check box, the value will be one unit higher than the low value, and one unit lower than
the high value.
7. Click Add Range if you want to add a new range. Type a Range Name, Low Value,
High Value, and check or clear the Inclusive check boxes.
8. If you want to delete a range, click a range to highlight it, and click Delete Range.
9. Check Mark as Intra-Well Normalization Bead if you wish to use a normalization
bead. The normalization bead is a microsphere set that is included in the assay as an
internal control. It controls for sample variation and can be used to normalize data
between samples in a run. An analyte used as a normalization bead will appear blue in
the analyte grid.
10.Type a name for the new formula in the Formula Name box.
11.Click Save Formula. The new formula displays in the Formulas list.
12.Click Apply to All Analytes to apply the new formula to all analytes in the list, or click
OK to apply the new formula to the single analyte you first clicked.
Create Quantitative Assay Protocol
The protocol must contain multiple standards. The standards are assigned lot value
information for each test. A standard curve is generated according to the lot values.
Including controls in the protocol is optional, but recommended for judging the
acceptability of batch results.
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Settings tab opens.
2. In the Name box, type the name of the protocol.
3. Type a description in the Enter optional description here box.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define settings in the Acquisition Settings section. For information about each of
these settings, see “Acquisition Settings” on page 25.
7. Define settings in the Analysis Settings section, selecting Quantitative as the
analysis type. The protocol for a quantitative assay must contain multiple standards.
Controls are optional in a quantitative assay. For information about the settings in this
section, see “Analysis Settings” on page 58.
8. Click Next. The Analytes tab opens.
9. Click the desired analytes (bead ID) in the numbered analyte grid. Information about
the analyte displays to the right side of the grid.
10.Click and type an analyte name in the Name column to the right of the analyte grid.
11.Click and type the desired unit of measurement in the Units box to the left of the Apply
All button.
12.Click and type the total desired bead count for each analyte in the Count box. Click
Apply All.
13.To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a bead count and units value.
14.To change the default analysis for all analytes, click Change. In the Analysis Settings
dialog box, select the analysis method from the Method list, and the weighting in the