Luminex xPONENT 4.0 SP1 - Manual (Page 136)

Luminex xPONENT 4.O Software Manual
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• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you wish to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose the desired command from the Command
list. Highlight the well that you want to apply it to, and then choose Before Well
Command or After Well Command. If you are working with more than one plate, click
Add Plate Select plates to display in the Plate list.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
• As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can also
import a protocol file by clicking Import List.
• Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.
16.Click Save.
Import Protocol
1. Open the Protocols page, then open the Protocols tab. Click Import.
2. In the Open dialog box, navigate to the protocol file you want to import, then click
Open.
3. The imported protocol displays in the Installed Protocols list.
Add New Lot for Protocol
1. Open the Protocols page, then open the Protocols tab. Click the protocol to which
you want to add a lot.
2. Open the Stds & Ctrls tab.
3. Click Create New Std/Ctrl Lots and select a protocol from the drop down list in the
Select Protocol dialog box, then click OK. The Std/Ctrl Details tab opens.
4. Click Apply Std/Ctrl Kit to associate a kit with the protocol. If you are not using a kit,
type the appropriate Standard and Controls information in the Assay Standard
Information and Assay Control Information sections.
5. Click Save.
Delete Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Delete. The Delete Protocol dialog box opens.
4. Click Yes.
Export Protocol
1. Open the Protocols page, then open the Protocols tab.
2. Select a protocol.
3. Click Export. The Save as dialog box opens.
4. Select a location to export the file to, and click Save.