Luminex xPONENT 4.0 SP1 - Manual (Page 18)

Luminex xPONENT 4.0 Software Manual
6
Reagents
Formulated reagents must be free of particulates other than xMAP beads. Do not dilute
xMAP calibrators or verifiers.
Gating
Gate positions are dependent upon buffer composition. Any changes made to the buffer
composition in an assay may result in a different optimal gate location.
Determine the gating on the Doublet Discriminator channel for the assay during assay
development. The numeric values appear on the left side of the histogram. Use the
numerical gate position, as determined during assay development, to set the gate location
in the protocol.
Gating information may change with a new lot of xMAP beads. Each time you receive a
new lot of xMAP beads, evaluate them with the current protocols. If gating information
changes, create a new protocol identical to the current protocol, but with a new version
number and new gating information. If running an xMAP-based kit, follow the instructions
found on the kit’s product insert or use the provided software protocol.
Plates
When using uncovered plates, use black opaque plates, if possible, to reduce
photobleaching.
For heated assays, use CoStar
®
Thermowell
®
96-well, thin-wall polycarbonate, model P
plates; Eppendorf
®
384-well plates, catalog number 951020737 or equivalent. For 384-
well heated assays, we also recommend the plate be covered with a pierceable foil plate
sealer.
For nonheated assays, select a 96-well plate or 384 well plate with an overall height no
greater than one inch (25.4 mm). See “Bead Concentration” on page 4.
See the recommended consumables list on the Luminex website at http://
www.luminexcorp.com/support/recommendedmaterials/index.html for more information.