Luminex xPONENT 4.0 SP1 User Manual

Page 34

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Luminex xPONENT 4.0 Software Manual

22

123123123. . . Lays out one of each replicate set at a time in numerical order.

111222333. . . Lays out all the replicates in a set before moving on to the next set in

numerical order.

You can assign the following well commands. Each command is associated with a color.
You can click and drag to highlight a series of wells, click a column or row header to
highlight the entire column or row, or simply click and highlight different wells and then
click a command below to assign that command to all the highlighted wells.

Unknown (U): Yellow

Background (B): Purple

Control (C): Red

Standard (S): Green

Wash (W): Blue

The Delete and Start at Well commands are also available to assign as well commands.
Delete removes the well command for the selected well. The Start at Well command
enables you to begin acquisition at a well other than A1.

NOTE:

You should first delete all standards from the plate layout if any of
the standards need to be rearranged, and all controls from the
plate layout if any of the controls need to be rearranged.

NOTE:

Wells and commands you assign to the protocol plate layout are
saved into the protocol settings and execute each time you use
the protocol to run a batch. Standards and controls associated
with a given protocol typically remain constant, while the number
of unknown wells often vary. You can assign a specific number of
unknown wells to the plate when setting up a batch.

Commands. Assigns maintenance commands that run before or after single wells, ranges
of wells, and rows or columns of wells. Select a well, and then select one of the following
commands:

Alcohol Flush

Prime

Sanitize

Soak

Wash

After selecting a maintenance command, click one of the following buttons:

Before Well - The system performs the command before acquiring the well. An open
dot appears in the upper left corner of the selected well on the plate image. The

command appears in the command sequence list before the selected well.

After Well - The system performs the command after acquiring the well. A closed dot
appears in the lower right corner of the selected well on the plate image. The command

appears in the command sequence list after the selected well.

Clear Command - Select a well and click Clear Commands to remove all
maintenance commands from the well.

NOTE:

If the batch has Before Well or After Well commands samples
will be run as in a standard syringe system, which will lengthen
the batch run time.

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