Luminex xPONENT 4.0 SP1 - Manual (Page 37)

25
Touring the Software
Name and Description boxes. Type a name and a description in the appropriate boxes.
Acquisition Settings. Use this section to set the following:
• Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads.
• Volume - This is the volume the instrument will aspirate into the system for analysis.
Type the desired sample volume in microliters. Use values from 10 to 200 µl for both
96-well and 384-well plates. To avoid air intake, add at least 25 µl to the sample well in
addition to the sample size. The default value is 50 µl.
• Timeout - Select Enabled to enable a timeout. Acquisition stops once the timeout is
reached. Type a value from 1 to 250 seconds.
• XY heater - Select Enabled to enable the XY heater. In the box, type the desired value
in degrees Celsius. The temperature range is 35 to 60°C in 0.5 increments.
WARNING: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• Plate Name - Click the Plate Name list and select Current 96-well plate, Current
384-well plate, or a plate that you have previously saved.
• DD gating - Use this to set the minimum and maximum DD gating values. Use values
within the range of 0 to 32767. In the first box, type the minimum value. In the second
box, type the maximum value. The default minimum and maximum values are 7500
and 13500 respectively.
• Reporter Gain - Select Standard PMT from this list to decrease the reporter gain
level. The default option is Enhanced PMT. Refer to assay application instructions for
more detailed information.
Analysis Settings. Use this section to set the analysis type, set the number of standards
and controls, select an external analysis program, and choose whether to analyze results
while acquiring samples.
• Analysis Type - Use this list to choose from the following analysis types:
• None - No analysis. Select if you have your own data post-processing program and
want to obtain only fluorescent intensity results. You cannot apply standards or
controls when you select None. You cannot analyze acquisitions with this setting.
• Qualitative - Qualitative analysis determines results as either positive or negative,
reactive or non-reactive. The software is flexible in defining custom result ranges,
such as negative, low positive, or high positive. Determinations are based on a
single standard. For qualitative analysis the Luminex software uses a specific
algorithm, shown below.
Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.
The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges
using the Lum Qual formula or an edited range specific for your assay, helps to
determine qualitative results for unknown samples.
Two predefined formulas using the algorithm are included in the system. You can use
them as is or edit their range values to meet your requirements. For information about
changing the analysis settings, see “Analysis Settings Dialog Box” on page 28 and
“Analysis Settings Dialog Box” on page 29.