Luminex xPONENT 4.0 SP1 User Manual
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Luminex xPONENT 4.0 Software Manual
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Median - The point in a data set where there are as many values occurring above it as
there are below it.
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Test Result - The calculated analysis value for quantitative or qualitative assays
derived from standards with known values.
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Range - A sem-quantitative result for a particular numerical result falling between a
predefined set of values such as “Normal” or “Negative”.
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Count - Gated events, if gates are set.
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Net MFI (Median Background) - Net MFI median background.
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Mean - Average of all values in a set of results.
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% CV of microspheres - The measure of relative dispersion within the distribution.
%CV = 100 x Std Dev / Mean
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Standard Deviation - For calculating sample variability or dispersion, Luminex uses
the standard deviation formula.
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Peak - The value that is equal to the largest number of data points within the
distribution. For example, in data set {1,2,2,3,3,3,4,5}, 3 is the peak because it occurs
the largest number of times in the distribution list.
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Trimmed Count*
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Trimmed Mean*
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Trimmed%CV of microspheres*
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Trimmed Standard Deviation*
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Trimmed Peak*
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% CV of Replicates - The measure of relative dispersion within the distribution of
results for replicate samples.
%CV = 100 X Std Dev / Mean
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% Recovery - A measure of how accurately your observed results match your
expected results following regression analysis.
(Observed concentration) / (Expected concentration) x 100%
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Expected Result - The known or expected test result value for a standard or control.
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Control Range - Low - The lowest value for an assay control used to determine pass/
fail criteria for an assay.
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Control Range - High - The highest value for an assay control used to determine
pass/fail criteria for an assay.
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Normalized Net Median - For each analyte in a well the Normalized Net Median
(NNM) = (net median of analyte) / (net median of normalization bead)
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Units - The unit of measure for an analyte, for example, pg/mL.
*Trimmed statistics remove the lower and upper five percent of the extreme statistic
values, then use the remaining values for the Mean, Standard Deviation, or %CV
calculations.
The statistics displayed change according to the analysis type selected.
Analyte. Contains a list of analytes run in the batch. Select an analyte to view all statistics
for that analyte.
Current Well. Displays the statistics of the well currently being displayed (This changes to
Displayed Well if viewing a batch using the Open button of the Saved Batches tab).
Single Step. Allows analysis one well at a time. Click to turn function on or off.