Luminex xPONENT 4.0 SP1 - Manual (Page 51)

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Touring the Software
Results. Displays statistics related to the batch. Use the up, down, left, and right arrow
buttons to move through the table, or use the scroll bars. The maximize/minimize toggle
button expands the batch table and returns it to the standard size.
Plate. A list of the available plates (if more than one).
WARNING: If using multiple plates, be careful to ensure that plates are used
in the proper order. Failure to do so can result in inaccurate data
and test results.
Histogram/Dot Plot. Displays real-time monitoring of batch sampling during acquisition.
Both the histogram and the dot plot have buttons that enable you to change the default
view:
• Click the maximize button to maximize the graph to fill the whole window. In the
maximized view, use the sliders to zoom in or zoom out. To return to the standard view,
click the minimize button.
If the histogram or dot plot display have been changed to another view (e.g. Progress,
Well Report), click Default to return to the default histogram and dot plot displays.
• The dot plot default display when using 1 to 100 beads shows Classification 1 and
Classification 2. When running more than 100 beads, the default is Classification 2
and Classification 3 axes, and radio buttons are displayed on the right for the
Classification 1 axis slices. To view the dot plot, you must use the default axes. To
display the bead set information, hover over the desired region. Click the upper view
classification button immediately below the maximize button to change the display to
show Classification 2 and Classification 3. This view displays a series of radio
buttons on the right side of the dot plot. When one of the radio buttons is clicked, the
bead regions in view are those in a fixed range of CL1 values plotted against CL2 and
CL3
. Click the bottom view classification button to change back to the default
classification display.
Histogram. The default location of the histogram is the lower left section of the Current
Batch tab. The Doublet Discriminator (DD) displays on the X axis. Events display on the
Y axis. Doublets appear when two beads stick together, creating undesired results. When
you select the gate, two vertical dashed red lines appear. These represent gate positions
determined by the protocol. Once the gate is set, everything outside of it is ignored. For
batches created using an existing protocol, the gate cannot be changed while running the
batch. For new batches created from new protocols, the gate can be changed while
running the batch. Any changes made to the gate while running new protocol batches
immediately affect the visual display of the dot plot and histogram. In addition, changes
made to the gate while running new protocol batches affect the acquisition for all wells
initiated after the change is made. When viewing saved batch data, the gate can be
changed to visually inspect the effect. Only the visual display of the dot plot and histogram
is affected for saved batches, not the acquired data.
NOTE: Gate positions are dependent upon buffer composition. Any
changes made to your assay buffer composition may result in a
different optimal gate location.
Right-click inside the histogram area to access the following options:
• X Axis - Use this function to change the X axis to the following: Doublet Discriminator,
Reporter 1, Classification 1, Classification 2, and Classification 3. Doublet
Discriminator is the default.