Luminex xPONENT 4.0 SP1 - Manual (Page 58)

Luminex xPONENT 4.0 Software Manual
46
Import. Opens the Open dialog box to choose a batch file (.mdf) to import. Select Include
Raw Files (LXB) to import the raw data file as well.
Export. Opens the Export Batch dialog box to choose a destination for the batch file
(.mdf). Select Include Raw Files (LXB) to include the raw file in the export. Select
Overwrite to overwrite pre-existing files.
Replay. Opens the Select Replay Mode dialog box.
FIGURE 29. Select Replay Mode Dialog Box
Replay batch - Uses the data stored in the run files from the initial acquisition to
reprocess a batch, creating a new batch output file. A batch can be reprocessed multiple
times.
NOTE: The initial batch data and output file always remain intact and
unchanged. Each time you replay a batch, the system handles it
as if it is a new batch, creating a separate batch entry and output
file.
Replay a batch to replay the raw bead data files. The bead data files will be replayed using
the gate, analyte, analysis settings, and plate layout selected in the new (or updated)
protocol. Settings such as bead type, volume, timeout, XY heater, and report gain will
have no effect.
Recalculate data - Reanalyzes the batch results using only the batch’s MFI values.
The batch MFI values will be recalculated using the analysis settings and plate layout
selected in the new recalculated batch or protocol. Settings such as bead type, volume,
timeout, XY heater, gate, report, and gain will have no effect. Because only the MFI values
are reanalyzed, no data will be displayed in the dot plot or histogram.
When you replay or recalculate a batch, you perform the same steps to create the batch
as you did when you created the batch the first time. This sequence will vary based on
whether you created a new batch from a new protocol or a new batch from an existing
protocol. The functionality of the tabs you see while configuring the replay batch is the
same as described in “Batches Tab” on page 16.