Luminex xPONENT 4.0 SP1 - Manual (Page 76)

Luminex xPONENT 4.0 Software Manual
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• Soak
• Wash
For information on what these commands do, see “Cmds & Routines Tab” on page 71.
After selecting a maintenance command, click one of the following buttons:
• Before Well - The system performs the command before acquiring the well. An open
dot appears in the upper left corner of the selected well on the plate image. The
command appears in the command sequence list before the selected well.
• After Well - The system performs the command after acquiring the well. A closed dot
appears in the lower right corner of the selected well on the plate image. The command
appears in the command sequence list after the selected well.
• Clear Command - Select a well and click Clear Commands to remove all
maintenance commands from the well.
NOTE: If the batch has Before Well or After Well commands, samples
will be run as in a standard syringe system, which will lengthen
the batch run time.
Plate. Specifies the plate you want to display in the plate image in the list. Add Plate adds
a new plate to the batch, and Delete Plate deletes the plate highlighted in the list.
Direction. Specifies the direction to run the plate commands. Select either horizontally or
vertically. The selected direction also dictates how wells are added to the plate when
assigning multiple unknowns, standards, and controls at one time.
Plate Navigation. Displays a smaller plate image for the current batch. If you have
selected a 384-well plate, there is a blue bounding box that can be clicked and dragged to
display the full range of wells in the larger plate image at the upper left.
Single Step. Instructs the system to acquire two wells and then stop. Use this to ensure
the system is working correctly before you run the whole batch.
Off Plate Area. Designates an alternate location for maintenance commands in the
Commands: list. Highlight a command, and then click either a reservoir or strip well in the
Off Plate Area to select a location for the command.
Cancel. Returns to the main Protocols tab without saving.
Back. Returns to the Analytes tab.
Save. Saves the protocol and returns to the main Protocols tab.