Luminex xPONENT 4.0 SP1 - Manual (Page 84)

Luminex xPONENT 4.0 Software Manual
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This tab contains the following:
Routine Name. This list of pre-set routines and commands can be used for system
maintenance. Some of these commands are also available on the Auto Maint tab.
Additionally, you can create custom routines on this tab, which display in the Routine
Name list once you save the routine. To create a custom routine, startwith None and add
the desired commands. You can also modify a Luminex routine and save it as a new
routine. Select from the following routines:
• Alcohol Flush (Luminex)
• Daily Instrument Shutdown (Luminex)
• Daily Instrument Startup (Luminex)
• Fluidics Maintenance (Luminex)
• None
• Prepare for Storage (Luminex)
• Remove Air Bubbles (Luminex)
• Remove Clog (Luminex)
• Revive After Storage (Luminex)
• Sanitize(Luminex)
• Weekly Maintenance (Luminex)
Plate Name. This list displays the available plates to use for the selected routine or
procedure.
WARNING: If using multiple plates users must be careful to ensure that plates
are used in the proper order. Failure to do so can result in
inaccurate data and test results.
Commands. This section contains the following commands:
• Wash - Sends distilled water through the fluidic lines in the system. It pulls the fluid
from a well or from the reservoir and runs it completely through the system to the waste
receptacle.
• Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking
the probe replaces sheath fluid in the probe with water. You should perform the soak
function at the end of each day. The system uses at least 250 µL of distilled water.
• F3DCAL1 - Calibrates DD, CL1, CL2, CL3 for original non-magnetic xMAP beads.
• F3DVER1 - Verifies DD, CL1, CL2, and CL3 for non-magnetic xMAP beads.
• Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using
70% isopropanol or 70% ethanol. The alcohol flush takes about five minutes. Alcohol
Flush uses the Luminex XYP reservoir because only the reservoir can accommodate
the amount of fluid necessary to flush the instrument.
• Warm-up - Warms the system to prepare the optics prior to sample acquisition. The
system automatically begins warming up when you turn power on. The process takes
thirty minutes. You will need to use the Warm-up command if the system is idle for four
hours or longer.
• F3DeCAL1 - Calibrates DD, CL1, CL2, and CL3 for MagPlex beads.
• F3DeVER1 - Verifies DD, CL1, CL2, and CL3 for MagPlex beads.
• Sanitize - Uses the off plate reagent area, because only the reservoir can
accommodate the amount of fluid necessary to sanitize the instrument. The sanitize
command performs a similar function as the alcohol flush command, but uses 10% to
20% household bleach and water solution to decontaminate sample lines and the