Luminex xPONENT 4.0 SP1 - Manual (Page 85)

73
Touring the Software
cuvette after biohazard contact. Sanitize as part of your daily shutdown routine after
biohazard contact.
• Prime - Removes air from the system’s fluidic pathways by drawing sheath fluid from
the sheath fluid container. You do not need to supply solution in a plate.
• F3DCAL2 - Calibrates xMAP reporters.
• F3DVER2 - Verifies RP1 at normal and high PMT and extended ranges for all xMAP
Bead types.
• Drain - Helps remove debris from the bottom of the cuvette during troubleshooting.
You do not need to supply solution. Draining takes approximately two minutes and
should be followed by an alcohol flush with 70% isopropanol or 70% ethanol. Any fluid
that drains from the system drains to the Luminex XYP reservoir as the default.
However, you can set the system to drain to any unused well on the microtiter plate.
The drain function normally expels 125 µL of fluid.
• Backflush - Removes obstructions from the fluidics pathways by drawing sheath fluid
from the sheath fluid container. You do not need to supply solution.
WARNING: Do not run three backflush commands in a row.
• F3DCAL3 - Calibrates extended RP1 ranges for all xMAP beads.
• Self Test - Performs a self-diagnostic to see if the system and all operations are
functioning correctly.
NOTE: You should perform a Self Test as part of your scheduled weekly
maintenance.
Click a command to add it to a new routine, to modify an existing routine, or to run the
single command. It will appear in the plate image to the right of the Commands. You can
also select a location first, which places the command where you choose. To change the
location of a command already on the plate, select it in the Command Sequence, and
then click a different well or reservoir in the plate image.
NOTE: When you modify an existing routine, by adding or clearing a
command, the routine name defaults to None in the Routine
Name dropdown menu and the Save As button appears.
Remember to save your new routine to make it available for future
use.
Command Sequence. When you select a routine, the individual commands in the routine
appear in order in the Command Sequence list. The list includes the name of each
command, location, status, and any additional information.
Clear. Clears the highlighted command in the Command Sequence list.
Clear All. Clears all commands in the Command Sequence list.
Report. Opens the Reports tab with the Calibration Summary selected in the Report:
list. Click Generate to view the selected report.
Import. Displays the Open dialog box to select a routine file to import. Select a file and
click Open to import it.
Export. Opens the Save As dialog box to specify a file name and location to save a
Routine file.