Adjusting the sample probe – Luminex xPONENT 3.1 Rev 2 User Manual

Page 109

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1. Type a company name in the Company box, and any additional information in the Info:

box.

2. Click Import Logo and then Open after navigating to the desired file to import a graphic

for the report header.

3. Click Clear Logo to remove the logo.

4. Click Save.

Adjusting the Sample Probe

Adjust the sample probe to ensure that it drops far enough into the well to acquire sample
without impacting the plate.

NOTE: Ensure that there is no liquid in the wells or reservoirs.

NOTE: You should adjust the probe height using row 1 unless you are using

the Automated Maintenance Plate. In that case, the probe
automatically moves to the specified well.

1. On the Home page, click Probe & Heater. The Probe & Heater tab opens.

2. Review the adjustment location for the well that you will use for alignment. Click a well on

the plate image if you want to use a different well. A blue pin notes the well selected for
alignment.

3. Remove the clear plastic shield that covers the sample probe area.

4. In a 96-well microtiter plate where overall height is no more than 0.75 inches (19mm),

place the appropriate alignment tool in the plate:

• For a standard plate with flat bottom wells - stack two of the larger (5.08 mm diameter)

alignment discs into the selected well.

• For a filter bottom plate - stack three of the larger (5.08 mm diameter) alignment discs

into the selected well.

• For a half-volume plate with flat-bottom wells - stack two of the smaller (3.35 mm

diameter) alignment discs into the selected well.

• For a round-bottom (U-bottom) plate - stack two of the smaller (3.35 mm diameter)

alignment discs into the selected well.

• For a plate with conical wells - place one alignment sphere into the selected well.

5. Verify that the microtiter plate is not warped. Warped plates can lead to incorrect probe

height adjustment

6. Click Eject to eject the plate holder. Place the plate on the plate holder with well A1 in the

top left corner.

7. Click Retract to retract the plate holder.

8. Loosen the probe adjustment screw on the probe holder one-third to one-half turn. Pull

the sample probe upward until it touches the top of the adjustment slide. Tighten the
probe adjustment screw.

9. Click Move Probe Down to lower the sample probe.

10. Gently loosen the probe adjustment screw. Push the probe down until it just touches the

top of the alignment discs or sphere.

xPONENT

®

3.1 Rev. 2

96

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