Luminex xPONENT 3.1 Rev 2 - Manual (Page 111)

Run System Initialization
1. On the Home page, click System Initialization. The Auto Maint page opens.
2. Select the performance verification kit from the Performance Verification kit list at the
right of the tab.
3. Select the calibration kit from the Calibration Kit list.
4. Vortex the xMAP calibrator, verification, and fluidics containers to ensure homogeneity.
Do not dilute xMAP calibrator or verification agents.
5. Click Eject on the status bar, then place the microtiter plate on the plate holder.
6. Load a microtiter plate with at least five drops of the beads in the Calibration and
Performance Verification kit as indicated on the plate image and in the kit insert
instructions.
7. Click Retract.
8. Click Run.
Add CAL or VER Kits
Before you can calibrate the system, you will need to add xMAP calibrator and verification
bead lot information. This information is on the CD that accompanies the performance
verification kit and is also available on the Luminex website at
http://www.luminexcorp.com/
Support/SupportResources/index.htm#tabs-4 .
WARNING: Inaccurate data entry can invalidate the results of these
procedures. Careful entry of data in each field is necessary to
ensure accurate results.
1. On the Home page, click System Initialization.
2. Click Import Kit at the bottom right side of the window. The Import Calibration or
Performance Kit dialog box opens.
3. Locate the xMAP calibrator or verification kit information file (.lxl) and click Open.
Import CAL or VER Kits
1. Open the Maintenance page, then open the Lot Management tab.
2. Click Import Kit. The Import Calibration or Performance Kit dialog box opens.
3. Select the kit you wish to import, then click Open.
Export CAL or VER Kits
1. Open the Maintenance page, then open the Lot Management tab.
2. In the Active Reagents section, select the kit you want to export from the Calibration
Kit or Performance Verification Kit lists.
3. Click Export.
4. In the Export Calibrator or Verification Lot dialog box, select an export location for the
kit.
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