Analyze current batch, Manage saved batches, Replay batch – Luminex xPONENT 3.1 Rev 2 User Manual

Page 118: Analyze current batch manage saved batches

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You can analyze an acquired batch using the analysis features of Qualitative, Quantitative,
and Allele Call algorithms.

Qualitative Analysis - Determines results as either positive or negative, reactive or non-
reactive, and so on. The system is flexible in defining custom result ranges, such as negative,
low positive, and high positive.

Quantitative Analysis - Determines the sample concentrations from standard curves using
regression methods, such as 4P or 5P logistic curve fitting. There are two main assay types:
non-competitive and competitive. In a non-competitive assay, the slope of a concentration
versus Median Fluorescent Intensity (MFI) standard curve is a positive number. That is, low
concentrations result in low MFIs and high concentrations result in high MFIs. Conversely,
competitive assays generate a standard curve with a negative slope, the endpoints of which
are high MFI/low concentration on the left, and low MFI/high concentrations on the right.

Allele Call - Compares groups of analytes in order to determine genotype. Groups can
include two, three, or four analytes for comparison. Set the call rate to determine what ratio
you want to set as the limit for the analyte comparison.

Analyze Current Batch

When you run a batch, the Current Batch tab opens. Here, you can view real-time analysis
of acquired analytes.

To view a single statistic type for all analytes click the statistic you want to view from the
Statistic list. To view all statistic types for a single analyte, click the analyte from the Analyte
list.

Click Single Step if you want to set the DD Gate during sample acquisition. Click Resume to
acquire the next sample. Clear Single Step if you want to run all subsequent samples without
pausing.

Manage Saved Batches

Replay Batch

You can replay a batch to reprocess batches multiple times. This function uses the data
stored in the run files from the initial acquisition to reprocess a batch and create a new batch
output file.

1. Open the Results page, then open the Saved Batches tab.

2. Click the batch you want to replay.

3. Click Replay. The Select Replay Mode dialog box opens. Click Replay batch.

4. In the Settings tab, type a batch name in the Batch Name box.

5. Type a description in the Description box.

6. Change other parameters as needed.

7. Click Next.

8. In the Analytes tab, change the Analyte, Default Analysis, Units and Count as

needed. Click Next.

Using the Software

105

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