Luminex xPONENT 3.1 Rev 2 - Manual (Page 125)

18. Select a weight type in the Weight Type list (Weight Type may not display, depending
on the analysis method selected in the Method list).
19. Check Mark as Intra-Well Normalization Bead if you want to use the analyte as a
normalization bead. The normalization bead is a microsphere set that is included in the
assay as an internal control. It controls for sample variation and can be used to normalize
data between samples in a run.
20. Check Use Threshold Ranges if you want to use a range for the analysis.
21. Click Add Range to setup the threshold range.
22. Type a name for the range in the Range Name field.
23. Type low and high range values in the Low Value and High Value fields.
24. Select the check box in the Inclusive column to include the value in the range, or leave it
clear to make the range value one unit higher than the low value, and one unit lower than
the high value.
25. Highlight a range and click Delete Range to delete the range.
26. Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes to
apply them to all the analytes in the protocol.
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