Luminex xPONENT 3.1 Rev 2 - Manual (Page 128)

9. Click the desired analyte (bead ID) in the numbered grid. Information about the analyte
appears in a list on the right side of the grid. For an allele call analysis, you must select a
group of two, three, or four analytes.
10. Click Group to group the analytes for the allele call. The grouped analytes display in a list
to the right. Select more analytes and then click Group again if you want to add another
group to the analysis.
11. Click and type an analyte name in the Name column to the right of the analyte grid.
12. In the Count box, type the desired bead count for each analyte. Click Apply All.
13. To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a units value and bead count.
14. Click in the Call % column and type a value to set an individual analyte’s call percentage.
15. Click Next. The Plate Layout tab opens.
• To add well commands, highlight the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added, and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose the desired command from the Command
list. Highlight the well that you want to apply it to, and then choose Before Well
Command or After Well Command. If you are working with more than one plate, click
Add Plate Select plates to display in the Plate list.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
• As you add commands to your plate, they appear in the Command Sequence list.
Type a well ID in the ID field. Here, you can give each of your wells an ID. You can
also import a protocol file by clicking Import List.
• Move commands up or down in the sequence by highlighting a command and using
the Move Command arrows to move the command up or down in the list.
16. Click Save.
Import Protocol
1. Open the Protocols page, then open the Protocols tab. Click Import.
2. In the Open dialog box, navigate to the protocol file you want to import, then click Open.
3. The imported protocol displays in the Installed Protocols list.
Add New Lot for Protocol
1. Open the Protocols page, then open the Protocols tab. Click the protocol to which you
want to add a lot.
2. Open the Stds & Ctrls tab.
3. Click Create New Std/Ctrl Lots and select a protocol from the drop down list in the
Select Protocol dialog box, then click OK. The Std/Ctrl Details tab opens.
Using the Software
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