Biological samples, Bead handling, Limitations – Luminex xPONENT 3.1 Rev 2 User Manual

Page 16: Safety precautions, Biological samples bead handling

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Use of unauthorized third-party software with xPONENT software may result in corruption or
failure of the xPONENT software. Use third-party software at your own risk.

If using a screen saver on the xPONENT system, be aware that xPONENT will prevent it
from activating. A dialog box will display each time xPONENT is launched, recommending
that the screen saver and any power management settings are turned off.

This system contains electrical, mechanical, and laser components that, if handled
improperly, are potentially harmful. In addition, biological hazards may be present during
system operation. Therefore, we recommend that all system users adhere to standard
laboratory safety practices. The protection provided by the equipment may be impaired or the
warranty voided if Luminex 100/200 systems are used in a manner not specified by the
instructions or by Luminex Corporation.

Biological Samples

Human and animal samples may contain biohazardous infectious agents. Where exposure to
potentially biohazardous material, including aerosol, exists, follow appropriate biosafety
procedures and use personal protective equipment - gloves, gowns, laboratory coats, face
shields, or mask and eye protection - and ventilation devices. Observe all local, state, and
federal biohazard handling regulations when disposing of biohazardous waste material.

Bead Handling

xMAP beads come in various configurations. Avoid excessive agitation of the product to
reduce foaming and surface precipitation. The xMAP beads will settle if left undisturbed.
Always ensure that the xMAP beads are homogeneously resuspended prior to dispensing.
The uncoupled xMAP beads are not monodispersed and tend to aggregate until coated.
Multiple pipetting from the original container may affect bead concentrations. Protect the
xMAP beads from light at all times. Store xMAP beads at 2 to 8°C.

NOTE: Refer to the product information sheet that accompanies your xMAP

beads or assay for additional information.

Limitations

xMAP beads are susceptible to photobleaching; photobleaching effects are cumulative.
Minimize exposure of xMAP beads to light to maintain product integrity.

xMAP beads are hydrophobic in the aqueous medium provided and will settle if left
undisturbed. Resuspend prior to dispensing.

Do not use this product with strong organic solvents. For information on specific compatibility,
visit the Luminex Technical Support website at

http://www.luminexcorp.com/support/

index.htm

Safety Precautions

All samples should be regarded as potentially contaminated and treated as infectious. These
samples should be handled at the Biosafety Level 2 as recommended for any potentially
infectious human serum or blood specimen in the Center for Disease Control/National
Institutes of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories” 1984.

Although beads do not contain hazardous or carcinogenic components at toxic levels, they
may be toxic if swallowed. Contact with acids liberates toxic gases. If product comes in
contact with skin, wash immediately with copious amounts of water. In case of accident, seek

Introduction

3

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