Luminex xPONENT 3.1 Rev 2 User Manual

Page 36

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Analysis Settings - Use this section to set the analysis type, set the number of standards

and controls, select an external analysis program, and choose whether to analyze results
while acquiring samples.

Analysis Type - Use this list to choose from the following analysis types:

None - No analysis. Select if you have your own data post-processing program and

want to obtain only fluorescent intensity results. You cannot apply standards or
controls when you select None. You cannot analyze acquisitions with this setting.

Qualitative - Qualitative analysis determines results as either positive or negative,

reactive or non-reactive. The software is flexible in defining custom result ranges,
such as negative, low positive, or high positive. Determinations are based on a single
standard. For qualitative analysis the Luminex software uses a specific algorithm,
shown below.

Where FI = Fluorescent Intensity and Ki = a “Quali” value entered in lot information to
determine the value or the qualitative assay standard.

• The “Quali” value determines a cutoff or threshold. This, in conjunction with ranges

using the Lum Qual formula or an edited range specific for your assay, helps to
determine qualitative results for unknown samples.

• Two predefined formulas using the algorithm are included in the system. You can use

them as is or edit their range values to meet your requirements. For information about
changing the analysis settings, see the “Analysis Settings Dialog Box” or the “Analysis
Settings Dialog Box” sections.

Quantitative - Determines the sample concentrations from standard curves using

regression methods Cubic Spline, Linear, Logistic 4P, and Logistic 5P, Luminex
Logistic 4P
, and Luminex Logistic 5P. Type the desired values for standards and
controls in the Number of Standards and Number of Controls boxes. Select either
Fit of All Standards or Mean of Replicates for the calculation of the curve fit.

NOTE: There are two forms of the 4P and 5P regression formulas. When

you change from one formula to another, xPONENT displays the
formula. The Luminex Logistic 4P regression is calculated as y = a +
b/(1 + (x/c) ^ d), while the widely used Logistic 4P regression formula
is calculated as y = a + ((b-a)/(1+(x/c) ^d)). The Luminex Logistic 5P
regression is calculated as y = a + (b/(1 + ((x/c) ^ d)) ^ f) and the
widely used Logic 5P regression fomula is calculated as y = a + ((b-
a)/(1 + ((x/c) ^ d )) ^ f).

NOTE: Luminex recommends Fit of All Standards as the most accurate

calculation of the curve fit .

Based on a range of quantitative, numerical results, a threshold range can be applied
to a quantitative analysis, for example, high, low, saturated, and expected.

Allele Call - Sets analysis for an allele call. Analytes must be put in groups of 2, 3, or

4 analytes.

Touring the Software

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