Luminex xPONENT 3.1 Rev 2 - Manual (Page 77)

Commands - This section contains the following commands:
• Wash - Sends distilled water through the fluidics lines in the system. It pulls the fluid from a
well or from the reservoir and runs it completely through the system to the waste
receptacle.
• Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking the
probe replaces sheath fluid in the probe with water. You should perform the soak function
at the end of each day. The system uses at least 250 µL of distilled water.
• CAL1 - Calibrates DD, CL1, and CL2 for original non-magnetic xMAP beads.
• CON1 - Verifies DD, CL1, and CL2 for original non-magnetic xMAP beads.
• Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using 70%
isopropanol or 70% ethanol. The alcohol flush takes about five minutes. Alcohol Flush
uses the Luminex XYP reservoir because only the reservoir can accommodate the amount
of fluid necessary to flush the instrument.
• Warm-up - Warms the system to prepare the optics prior to sample acquisition. The
system automatically begins warming up when you turn the power on. The process takes
about thirty minutes. You will need to use the Warm-up command if the system is idle for
four hours or longer.
• MagCAL1 - Calibrates DD, CL1, and CL2 for MagPlex beads.
• MagCON1 - Verifies DD, CL1, and CL2 for MagPlex beads.
• Sanitize - Uses the off plate reagent area, because only the reservoir can accommodate
the amount of fluid necessary to sanitize the instrument. The Sanitize command performs
a similar function as the alcohol flush command, but uses 10% to 20% household bleach
and water solution to decontaminate sample lines and the cuvette after biohazard contact.
Sanitize as part of your daily shutdown routine after biohazard contact.
• Prime - Removes air from the system’s fluidics pathways by drawing sheath fluid from the
sheath fluid container. You do not need to supply solution in a plate.
• CAL2 - Calibrates xMAP reporters (RP1).
• CON2 - Verifies RP1 at normal and high PMT for all xMAP bead types.
• Drain - Helps remove debris from the bottom of the cuvette during troubleshooting. You do
not need to supply solution. Draining takes approximately two minutes and should be
followed by an alcohol flush with 70% isopropanol or 70% ethanol. Any fluid that drains
from the system drains to the Luminex XYP reservoir as the default. However, you can set
the system to drain to any unused well on the microtiter plate. The drain function normally
expels 125 µL of fluid.
• Backflush - Removes obstructions from the fluidics pathways by drawing sheath fluid from
the sheath fluid container. You do not need to supply solution.
• Self Test - Performs a self-diagnostic to see if the system and all operations are
functioning correctly.
NOTE: You should perform a Self Test as part of your scheduled weekly
maintenance.
Click a command to add it to a new routine, to modify an existing routine, or to run the single
command. It will appear in the plate image to the right of the Commands. You can also
select a location first, which places the command where you choose. To change the location
of a command already on the plate, select it in the Command Sequence, and then click a
different well or reservoir in the plate image.
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