Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 105)

Term Definition
dot plot Graphical representation of microsphere analysis
classification data displayed by frequency and
intensity.
doublet discriminator (DD) A classification channel measuring side scatter;
used to filter for single bead events based on
size.
emission spectrum Wavelength range that an excited fluorochrome
emits when its electrons fall from a higher to a
lower energy state. Expressed in nanometers
(nm).
event Occurs when the signal processor determines
that a particle of any size has passed through the
lasers.
excitation spectrum Wavelength range that excites a molecule’s
electrons to a higher energy state. Expressed in
nanometers (nm).
fluorescence Light emission that occurs when the electrons of a
fluorochrome drop to a lower energy state.
fluorochrome A fluorescent molecule.
fluorophore See fluorochrome.
histogram The representation of frequency distribution for
analyte results. The DD channel results are often
displayed in a histogram format.
immunofluorescence A technique which uses a covalently linked
fluorochrome-antibody complex to detect or
quantify a particular antigen.
kit A group of standards and controls used to create
a protocol. They are used in the protocol’s
definition for the multi-analyte assays that will be
performed. Formerly called “product” in previous
releases of Luminex software.
Luminex xMAP microsphere set Luminex multi-analyte microspheres containing a
unique mixture of two distinctly colored
fluorochromes to distinguish them from other
multi-analyte microspheres.
laser Light Amplification by Stimulated Emission of
Radiation. This highly purified source of light is an
efficient way to excite fluorochrome electrons.
LIS Laboratory Information System; A system for
storing sample records and test requests.
lot A reagent with specific target values used for
analysis or calibration.
median fluorescence intensity (MFI) Used to denote how much fluorescence a given
particle population carries; expressed and
compared in linear numbers.
TABLE . Continued
For In Vitro Diagnostic Use Glossary
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