Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 107)

Term Definition
reporter A molecule (or combination of molecules) with a
specific range of excitation and emission
wavelengths that is used to identify or quantify an
analyte. Examples of acceptable reporters are
Phycoerythrin and Alexa 532. Reporter signals
are the final result of each assay.
reporter channel (RP or RP1) A specific range of wavelengths that includes the
emission wavelength of a designated reporter
molecule.
RP See reporter channel.
RP1 Refers to the dyes bound to the surface of the
xMAP microsphere. Also see reporter channel.
sample The mixture of assay components (microspheres,
reporter, patient dilutant) that are analyzed.
sample probe The probe, or needle, on the Luminex analyzer
that acquires sample from the 96-well plate.
sample reaction The reaction that occurs between reagents and
beads.
sheath fluid Luminex xMAP Sheath Fluid is the delivery
medium of the sample to the optics component.
Only Luminex-approved sheath fluid should be
used when operating the Luminex analyzer.
signal Detectable measurement unit of the reporter
molecule.
standards microspheres, assay Assay standards are substances of known
concentrations used to derive a standard curve
with which unknown samples and controls are
compared to determine their concentration or
quantity.
suspension Solution consisting of homogeneously dispersed
microspheres in an aqueous medium.
system controls Include the xMAP reporter and classification
control microspheres. They are used to verify the
calibration of the Luminex analyzer.
template This is now called a protocol.
test Each test represents an analyte and corresponds
to a bead set. Each bead set falls into a particular
location on the bead map.
total event Everything passing through the cuvette and
analyzed by the instrument. This includes single
beads, aggregates of beads, debris, and air
bubbles.
verification The process using system controls to ensure the
analyzer is functioning properly with current
calibration settings.
TABLE . Continued
For In Vitro Diagnostic Use Glossary
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