Run system initialization, Add cal or ver kits, Run system initialization add cal or ver kits – Luminex xPONENT 3.1 Rev 2 (IVD) User Manual
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• Open the Maintenance page, and then the Cmds & Routines tab. Click Warmup in the
Commands section at the left of the screen, and then click the Run button at the bottom
right to begin the procedure. The warmup process takes 30 minutes to complete.
Calibrator xMAP beads are used to normalize the settings for the reporter channel, both
classification channels, and the doublet discriminator channel. Verification xMAP beads are
used to verify calibration and optical integrity of the system.
NOTE: When you run MagPlex microspheres, it is essential to use both
regular calibration reagents (CAL1 and CAL2) and magnetic
calibration reagents (MagCAL1) every time the system is calibrated.
Both must pass for the system to be fully calibrated and ready for
running magnetic beads.
Once calibrated, the values remain until you recalibrate. You can track system calibration and
verification results through the Calibration report. Target value information for calibration and
verification beads is available on the Luminex website at
.
Calibrate your system at least once a week using the Calibration/Verification button on the
Auto Maint tab of the Maintenance page. In addition, recalibrate the system if the delta cal
temperature exceeds +/-3º C. Verify the system daily using the Performance Verification
button on the Auto Maint tab of the Maintenance page. Refer to your assay kit instructions
for additional calibration frequency requirements.
Alternatively, you can set up the system initialization routine to include calibration and or
verification under the Admin page, System Setup tab, Maintenance Options section.
Ensure that the Luminex analyzer lasers are warmed up and the probe height is set correctly
before calibrating the system. Do not move the system waste line while calibrating.
Run System Initialization
1. On the Home page, click System Initialization. The Auto Maint page opens.
2. Select the performance verification kit from the Performance Verification kit list at the
right of the tab.
3. Select the calibration kit from the Calibration Kit list.
4. Vortex the xMAP calibrator, verification, and fluidics containers to ensure homogeneity.
Do not dilute xMAP calibrator or verification agents.
5. Click Eject on the status bar, then place the microtiter plate on the plate holder.
6. Load a microtiter plate with at least five drops of the beads in the Calibration and
Performance Verification kit as indicated on the plate image and in the kit insert
instructions.
7. Click Retract.
8. Click Run.
Add CAL or VER Kits
Before you can calibrate the system, you will need to add xMAP calibrator and verification
bead lot information. This information is on the CD that accompanies the performance
.
xPONENT
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3.1 Rev. 2 Software User Manual
76
For In Vitro Diagnostic Use