Luminex xPONENT 3.1 Rev 2 (IVD) - Manual (Page 90)

1. Open the Batches page.
2. Click Create a New Batch from a new Protocol to open the Settings tab.
3. Type a name in the Name box.
4. Type a description in the Description box.
5. Define the settings in the Acquisition Settings section.
NOTE: For information about each of these settings, see the Settings Tab
section.
6. Define the settings in the Analysis Settings section. For more information about each of
these settings, see the Analysis Settings information within the Settings Tab section. If
you select Quantitative or Qualitative from the Analysis Type list, you can select
Analyze results while acquiring samples to view real-time analysis. The xPONENT
Analysis Package can only analyze batches acquired using qualitative or quantitative
protocols.
7. Click Next. The Analytes tab opens.
8. Click the desired analyte in the numbered grid. Information about the analyte displays in
a list on the right side of the grid.
9. To change the Default analysis, click Change. The Analysis Settings dialog box
opens.
10. Select the analysis method from the Method list. Click OK to change the default analysis
for analytes to be selected. Click Apply to All Analytes to apply the selection to all
analytes. The Analysis dialog box closes.
11. Type the units of measurement you want to use in the Units box. If you click Apply All,
this choice is applied to all analytes. To change individual units, change them in the
analyte table in the dialog box.
12. Type the desired bead count for each analyte in the Count box. If you want to change
individual bead counts, change them in the analyte table in the dialog box.
13. Click Next. The Stds & Ctrls tab opens . This tab does not open if no analysis is
selected.
14. If you are using an assay standard/control kit, click Apply Std/Ctrl Kit. In the Select Std/
Ctrl Kit dialog box, click the kit from the list and click OK. Applying a kit only works for
kits already installed, however, you can manually type the information.
15. If you are not using a kit, type the appropriate information in the Standard Information
and Control Information sections. The number of standards and/or controls in these
sections is defined on the Settings tab in the Analysis Settings section. If your batch
uses controls enter the high value for each analyte. Reagent information is not required
for a custom batch unless you want to use the analysis feature.
16. Click Next. The Plate Layout tab opens.
• To add well commands, click the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose the desired command from the list. Choose
the well that you want to apply it to, and then choose Before well command or After
xPONENT ® 3.1 Rev. 2 Software User Manual
80
For In Vitro Diagnostic Use