Luminex xPONENT for MAGPIX - Manual (Page 110)

Plate Name - The name assigned to the plate.
CAUTION: If using multiple plates users must be careful to ensure that
plates are used in the proper order. Failure to do so can result in
inaccurate data and test results.
Commands - This section contains the following commands:
• Wash - Sends 250µl distilled water through the chamber and fluidic lines in the system. It
pulls the fluid from a well or from the reservoir and runs it completely through the system to
the waste receptacle.
• Soak - Prevents clogs in the probe, chamber, and sample loop. This command uses 200µl
of DI water from an off-plate reservoir; the system expels fluid into the system waste
container.
• Rinse - Re-primes the probe and rinses out the chamber. You do not have to add any
reagent. One of the off-plate reservoirs must be empty because the system will expel fluid
into the reservoir.
• CAL - Calibrates the LEDs and focuses the camera.
NOTE: When you choose the CAL command, a Rinse command is
performed prior to the CAL. When the CAL is complete, two
additional Rinse commands are performed. This prevents a bubble
from loading into the chamber. Verify that you have sufficient room in
the reservoir to complete these Rinse commands.
• Alcohol Flush - Removes air bubbles from the fluidics line and chamber using 70%
isopropanol or 70% ethanol. This command uses 100µl of alcohol from the off-plate
reagent block reservoir.
• Prime - Removes air from the system’s fluidic pathways by drawing Drive Fluid from the
Drive Fluid container. You do not need to supply solution in a plate.
• VER - Verifies the system is performing to specifications.
NOTE: When you choose the VER command, a Rinse command is
performed prior to the VER. When the VER is complete, an
additional Rinse command is performed. This prevents a bubble
from loading into the chamber. Verify that you have sufficient room in
the reservoir to complete these Rinse commands.
• Sanitize - The sanitize command performs a similar function as the alcohol flush
command, but uses 100µl of 10% to 20% household bleach and water solution to
decontaminate sample lines after biohazard contact. Sanitize as part of your daily
shutdown routine after biohazard contact.
• Self Test - Performs a self-diagnostic to see if the system and all operations are
functioning correctly.
NOTE: You should perform a Self Test as part of your scheduled weekly
maintenance.
Fluidics - Runs fluidics to check well-to-well carryover.
Performing System Maintenance
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