Luminex xPONENT for MAGPIX - Manual (Page 20)

when the analyzer flushes the sample lines after sample acquisition and expels
approximately 75 µL of sample back into the well.
Examples
• If you use a sample volume of 50 µL and aspirate 50 µL, you will acquire air bubbles.
• If you use a sample volume of 200 µL and a standard sample pickup of 50 µL, the well will
overflow when the analyzer washes the sample lines after acquisition and expels fluid back
into the well, because the amount of fluid expelled back into the well is approximately 75
µL.
CAUTION: Sample volume is critical to the proper functioning of your
MAGPIX instrument. Aspirating too few beads can result in
insufficient bead count or insignificant data results. Aspirating too
many beads can result in saturation of the chamber and prevent
proper bead classification, which may also result in low bead
counts or inconclusive data.
Follow the instructions in the IVD kit’s product insert and use the software protocol provided.
Do not dilute MagPix Calibration or Verification beads, or the Fluidics 1 and Fluidics 2 beads.
Plates
Follow the xMAP-based IVD kit instructions for use. Follow these guidelines when choosing
plates:
• When using uncovered plates, use black opaque plates to reduce photobleaching.
• For heated assays, use CoStar ® Thermowell ® 96-well, thin-wall polycarbonate, model P
plates.
• For unheated assays, use a 96-well plate with an overall height no greater than 0.75
inches (19 mm).
WARNING: The heater plate of the MAGPIX plate carrier may be hot. Do
not touch the heater plate.
See the recommended consumables list on the Luminex website at http://
www.luminexcorp.com/support/recommendedmaterials/index.html for more information.
Touch Screen
A touch screen option is available with xPONENT for MAGPIX.
Introduction
7