Multichannel Systems HiClamp update-2.95 Manual - Manual

HiClamp Software – What is New in Version 2.95
Installation
Please deinstall the “old” HiClamp software before installing the new version by using the deinstall function
from the Windows control panel. Backup the HiClamp program folder before starting the deinstallation.
Execute the setup file “HiClamp_Setup-1.2.95.exe” to install version 2.95. Use the setup file
“HiClamp_Setup-1.2.95_Full.exe” only if you want to install the software on a new computer.
Software version 2.95 works on the basis of some important changes in how the software communicates with
the amplifier. To benefit from these changes it is obligatory to perform an amplifier firmware update to
version 0.56 before using the software. Please use the program “HiClamp Firmware Update” the icon of
which will appear on your desktop after execution the setup file.
Important note: Please do not switch off computer or HiClamp while the firmware update is running.
Also plug in the power adapter from the laptop before starting the firmware update.
Preferences
You can change now the value for the oocyte membrane potential
(Cell(mV)) which is used as threshold during the automatic oocyte
impalement. This feature is particularly useful for testing new
protocols with the test model cell: type in a positive number (e.g.
15 mV) and you can run complete protocols without modification
(i.e. without deleting the oocyte icon).
Please do not forget to change it back to a negative value before
using oocytes. Choosing the “right” value can also increase the
number of successful recordings.
Amplifier Window
There are visible changes in the amplifier window but - more
importantly – also changes during the impalement procedure:
The CX_offs value is now modified automatically before every
oocyte impalement until the measured CX_CAP value is identical
to CX_Opt. The CX_Opt value is 230 by default and should not
be changed by the user.
Gain values 400 and 100 for gain and I-gain respectively are
default values for ligand-gated ion channels. Together with a low
filter frequency (e.g. 50 Hz) these values minimize the
occurrence of artefacts during basket movements (glitches) but
guarantee a time resolution sufficient for recording of ligand-
gated channels, where the response is elicited by agonist
application.
For recording of voltage-gated channels (i.e. using voltage steps
for channel activation) higher gain values and higher filter
frequencies may be needed for having a sufficient time-
resolution.
Parameters for “Stability” and “Series Comp” are 0 by default and should not be changed unless you are told
so upon future software updates or by the MCS support team.
Do not hesitate to contact MCS if you need further assistance.